Bjellqvist B, Basse B, Olsen E & Celis JE, 1994

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Bjellqvist et al. report a nearly complete set of amino acid pK values as part of their work in analyzing 2D gel electrophoresis (1). The pK values they report are pK values for the amino acids in polypeptides in 9.8 M urea at 25 oC. The pK values reported here are based on measurements and calculations from a variety of other studies (2-6).

Amino acid pK values from Bjellqvist B, Basse B, Olsen E & Celis JE, 1994 (1).

Group

3.55

†

ASP

4.05

†‡

GLU

4.45

†¶

HIS

5.98

†

7.42

†§

CYS

†

TYR

†

LYS

†

ARG

†

† Data from Table 1.

‡ Bjellqvist et al. also report an aspartic acid sidechain pK when the aspartic acid is found at the c-terminal end of the peptide, 4.55.

¶ Bjellqvist et al. also report a glutamic acid sidechain pK when the glutamic acid is found at the c-terminal end of the peptide, 4.75.

§ This value is the average of the eight (8) n-terminal pK values reported, ALA, 7.59; GLU, 7.70; GLY, 7.50; MET, 7.00, PRO, 8.36; SER, 6.93; THR, 6.82; VAL, 7.44.

References

(1) Bjellqvist B, Basse B, Olsen E & Celis JE (1994) Reference points for comparisons of two-dimensional maps of proteins from different human cell types defined in a pH scale where isoelectric points correlate with polypeptide compositions. Electrophoresis 15:529-39.

(2) Bjellqvist B, Hughes GJ, Pasquali C, Paquet N, Ravier F, Sanchez JC, Frutiger S & Hochstrasser D (1993) The focusing positions of polypeptides in immobilized pH gradients can be predicted from their amino acid sequences. Electrophoresis 14:1023-31.

(3) Perrin DD, Dempsey B, & Serjant EP (1981) pKa predictions for organic acids and bases, Chapman and Hall, Ltd., London

(4) Perrin DD (1965) Dissociation constants of organic bases in aqueous solution, Butterworths, London

(5) Perrin DD (1972) Dissociation constants of organic bases in aqueous solution: supplement 1972, Butterworths, London

(6) Altland K, Becher P, Rossmann U & Bjellqvist B (1988) Isoelectric focusing of basic proteins: the problem of oxidation of cysteines. Electrophoresis 9:474-85.